Figure 2

Chromosomal and plasmid synteny plots. Panels 1a-d or 2 a-d show four synteny plots of either chromosomal or plasmid sequence alignments, respectively. The reference sequence listed on the x-axis was queried with the strain sequence listed on the y-axis. The red dots indicate forward matches of the sequence comparisons: the blue dots indicate reverse compliment matches. The continuous diagonal line in the plot in panel 1a illustrates the overall chromosomal organization or synteny shared between the proteolytic strains of Hall and either the Kyoto-F, Loch Maree, 657, Okra or Langeland strains. Panel 1b and 1c plots compare Hall and C. butyricum BL 5262 to the BoNT/E-producing Alaska E43 strain, where little synteny is observed. In panel 1d four contigs of C. sporogenes ATCC 15579 are compared to the Hall strain and reveal genomic synteny and a 701 kb inversion between the two species. Panels 2a-d examine plasmid synteny. The diagonal lines in panel 2a illustrate that the Loch Maree pCLK has a similar organization to pCLJ with a small 16.7 kb inversion that includes the bont/a3 relative to the bont/a4. Panels 2b and 2c show that pCLL within Eklund 17B does not share synteny either to pCLK or pE88 that contains the tetanus toxin. In panel 2d four contigs of the Bf strain show synteny to pCLJ and the 16.7 kb inversion of bont/a4 relative to the bont/f.