Figure 1

Variants of rainbow trout finTRIM ORFs. (A) Schematic representation of the structure of the rainbow trout finTRIMs from SSH and cDNA library. ORFs were predicted from the sequence of full-length transcripts. The RING, B1 and B2 boxes, coiled-coil and PRY/SPRY domains are represented by boxes with specific colors. (B) Quantitative RT-PCR analysis of finTRIM induction in trout leukocytes (Leuk.) incubated with the virus (VHSV) or LPS for 40 h or in RTG-2 fibroblasts (Fibr.) incubated with poly(I:C) for 24 h, respectively. Normalized values for finTRIM expression were determined by dividing the average finTRIM value by the average β-actin value. Then, normalized finTRIM values were subjected to a calibration relative to the basic expression in control (Ctrl) leukocytes or RTG2 cells. The error bars represent standard deviation. (C) Complex profiles produced by 3'RACE amplification of rainbow trout finTRIMs from leukocytes incubated with (V) or without VHSV (Ctrl). (D) 3'RACE profiling of finTRIM from fibroblasts incubated with poly(I:C) (I:C) or without poly(I:C) (Ctrl). (E) Structure of the rainbow trout finTRIM ORFs from the 3'RACE products. Five classes could be distinguished on the basis of motifs and ORF lengths. Striped boxes indicate regions without similarity to the finTRIM consensus, perhaps corresponding to alternative splicing to additional exons. The number of clones corresponding to the different classes from the fibroblasts incubated with poly(I:C), or from leukocytes incubated with or without VHSV are indicated on the right.