Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

Table 2 Chromosomal location of lethal piggyBac insertions

Chromosome	Insertions	Chromosome size* [Mb]	Insertions/Mb
X	19	10.9	1.7
2	31	20.2	1.5
3	67	39.0	1.7
4	30	13.9	2.2
5	31	19.1	1.6
6	18	13.2	1.4
7	33	20.5	1.6
8	22	18.0	1.2
9	27	21.5	1.3
10	13	11.4	1.1
unmapped	14

305 insertions were localized in the genome sequence. Of these, 14 were on unmapped scaffolds and 11 could be assigned to chromosomes, but not to the exact position. The distribution of the remaining 280 lethal insertions in the genome is shown in Figure 3.
*based on NCBI map viewer, build 2.1.

ISSN: 1741-7007