Phylogenetic analysis of the arthropod ASH and Ase-like proteins. ASH and Ase of insects group together, as do those of crustaceans, while the ASH proteins of myriapods and of chelicerates are arranged in a single group. Both the insect and the crustacean proteins are clearly subdivided into ASH and Ase groups. The spider proteins CsASH1 and CsASH2 are arranged in a group with the single myriapod orthologues. Cs, Cupiennius salei; Dm, Drosophila melanogaster; Dp, Daphnia pulex; Gm, Glomeris marginata; Sm, Strigamia maritima; Tc, Tribolium castaneum.