C. salei CsASH2 and T. castaneum ase can both rescue the bristle phenotype in D. melanogaster ase1 mutants. (A) Bar chart showing the extent to which Dm-ase, Tc-ase and CsASH2 can rescue the phenotype when ectopically expressed (hsp70Gal4 > UAS Dm-sc/Dm-ase/Tc-ase/CsASH2). In contrast, Dm-sc enhances the ase1 phenotype (hsp70Gal4 > UAS Dm-sc). The number of bristles affected is given on the x-axis. Error bars indicate the standard error of the mean (see Additional file 1 for details). (B-G) Abnormally differentiated bristles (asterisks) on the anterior wing margin are shown for the genotypes indicated (genotypes as in (A)). The number of abnormal bristles is enhanced in (D) and reduced in (F, E, G). (H-K) Detail of the abnormalities at higher magnification.