Figure 9

Patterns of MyoB and of the Arp2/3 complex around the neck of a budded yeast. (a) GFP-MyoB (green) and mRFP-ArpC1 (red) during partial uptake and release of the particle. The labels remained enriched for 3 minutes during arrest of the phagocytic cup at the neck of the particle and disappeared before the particle was released. For the complete series see Additional file 8. (b) One frame from the same time series as in (a), showing the fluorescences of GFP-MyoB (top) and mRFP-ArpC1 (bottom) in separate channels. The colored line indicates the scan position in (c). (c) Quantification of fluorescence distributions of MyoB (green) and ArpC1 (red) along the line scan denoted in (b). The highest values of fluorescence intensity are set to 100. Time is indicated in seconds after the first frame in (a). Bars = 10 μm.