Methylation status of the Dlc1 alternative isoform promoters in different mouse tissues. (A) The Dlc1 6.1 and 6.2 isoform promoter sequences showing the location of the polymerase chain reaction primers (underlined), sequencing primer (bold and italics) and the CpG islands (numbered) that were analysed. (B) The reference peak heights for the 6.1 (left) and 6.2 Kb (right) isoform promoter sequences. (C) Pyrograms for liver DNA, showing the 6.1 Kb isoform promoter (left) and the 6.2 Kb isoform promoter (right). (D) The mean percentage of methylation for the different CpG islands in the mouse tissues examined, 6.1 Kb promoter (left), 6.2 Kb promoter (right).