Figure 7

Multiplex reverse transcriptase polymerase chain reaction (RT-PCR) showing the effect of gene trap insertion on the various Dlc1 isoforms. (A) Diagrammatic representation of the Dlc1 gene showing the position of different PCR primers sets used; (i) to show relative expression of GAPDH mRNA and Dlc1 isoforms and (ii) to show the relative expression of gene trap fusion transcripts and Dlc1 normal transcripts. (B) Multiplex RT-PCR showing the expression of 7.6 Kb, 6.2 Kb and 6.1 Kb Dlc1 isoforms and alternative splice forms relative to GAPDH expression (C) Multiplex RT-PCR showing the expression of 7.6 Kb, 6.2 Kb and 6.1 Kb Dlc1 isoform normal transcripts relative to their fusion splice products with βgeo of the gene trap vector.