Primers for 3'RACE | Â | Â | Â |
---|---|---|---|
1st strand CDNA synthesis | CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCTTTTTTTTTTTTTTTTT | ||
 | Forward primers | Reverse primers | Sequencing primers |
1st round PCR | CTATCAGAAAGAGGAGCTGGGAAG AGACCGAACAGCCTTTTATTTCTG ACGTCTTAGCAGCTCCTTATGTAG ACAAAAGCACTCTCGGGGTCAG AAACTGGACCAACTAGACCAAGAC ATCTGAATTGCACAGGCAAAACAG GCCCTAAGCCTTTCCAGATG CTGCGCCGACCTTAATGTGTAG | CCAGTGAGCAGAGTGACG | Â |
2nd round PCR | CTATCAGAAAGAGGAGCTGGGAAG AGACCGAACAGCCTTTTATTTCTG ACGTCTTAGCAGCTCCTTATGTAG ACAAAAGCACTCTCGGGGTCAG AAACTGGACCAACTAGACCAAGAC ATCTGAATTGCACAGGCAAAACAG TCCTTCAGCGACCACATCAGAG CGCCGACCTTAATGTGTAGAGAC | GAGGACTCGAGCTCAAGC | ATTTTTTTTTTTTTTTTTTTTT GTTTTTTTTTTTTTTTTTTTTT CTTTTTTTTTTTTTTTTTTTTT & different forward primers |
Primers for Northern probe | |||
1st round PCR | CTGCGCCGACCTTAATGTGTAG | GTTGCAGTCACGGGTGCTTC | Â |
2nd round PCR | CTCGAGCTGCGCCGACCTTAATGTGTAG | GTCGACGTTGCAGTCACGGGTGCTTC | Â |
Primers for real time quantification of splice variants | |||
7.6 Kb transcript | TCCACCGATCCATCATCCACTC | CGGTGAGGACTGATCTCCAGCTTCATG | Â |
6.2 Kb transcript | TCCTTCAGCGACCACATCAGAG | GACTGGTTTCCCCCAGTATGCA | Â |
6.1 Kb transcript | CGCCGACCTTAATGTGTAGAGAC | Â | Â |
2.1 Kb transcript | TCCACCGATCCATCATCCACTC | GCCCACTGAGTGCTGAGACTG AAGTAC | Â |
GAPDH | TTC CGT GTT CCT ACC CCC AAT GTGT | GGAGTTGCTGTTGAAGTCGCAGGAG | Â |
Primers for methylation study | |||
6.1 Kb Transcript | Biotin-GTAAGAAAAGGTAGAGGGGAAATTGAGTA | CTCCTTCCCCCTTTCCTAAAATAT | TTTTCCCAAAATCTACACT |
6.2 Kb transcript | TAGGAGGTTAGTATGGGTTGTA | Biotin-AAACCTAATACTAACATAAAACAATATC | AAATTGTTTTTTTGATTTTAA |
Multiplex PCR primers for genotyping Dlc1 gene trap embryos | |||
 | TCCTGCTGGTGTGTGTAAACAGAC | GTGTAAGTTTGCCTCCTCCTTTGG |  |
 | GATTCCACCGCCGCCTTCTATG | TCAAGCAAAAAGGTCTCGTATGTTGTG |  |
Multiplex PCR primers for fusion transcripts | |||
(7.6+2.1)Kb transcript | TCCACCGATCCATCATCCACTC | ACGACGACAGTATCGGCCTCAG | Â |
6.2 Kb transcript | TCCTTCAGCGACCACATCAGAG | Â | Â |
6.1 Kb transcript | CGCCGACCTTAATGTGTAGAGAC | Â | Â |