Figure 7
From: ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function
Interaction of human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein (ASB9) with ubiquitous mitochondrial creatine kinase (uMtCK). (a) Schematic diagram of the ASB9 and a series of ASB9 N-terminal ankyrin (ANK) deletion mutants. (b) ANK repeats of ASB9 are required for uMtCK interaction. Myc-tagged ASB9 and a series of ANK deletion mutants were transiently cotransfected with haemagglutinin (HA)-tagged uMtCK into human embryonic kidney 293 (HEK293) cells. Cell lysates and an immunocomplex of the anti-Myc antibody were analysed by immunoblotting with anti-Myc or anti-HA antibodies.