Figure 6
lgl-/- malignant behaviour in a Minute background depends on dMyc protein levels. A-C"': lgl-/- clones (GFP-) in a w, hs-Flp/+;l(2)gl4, FRT40A/M(2)24F, Ubi>GFPnls, FRT40A background (GFP+). A: dMyc and aPKC staining. A strong dMyc accumulation is visible in the part of the mutant clone located in the hinge region (arrows). A Z projection of the mutant clone shows that loss of cell polarity accompanies the dramatic overgrowth; the apical-basal axis of the disc proper is shown. A Z projection of a region of the disc without mutant clones is shown on the right as a control. B: Activated Caspase 3 and dIAP1 staining. Arrows indicate M/+, lgl+/- cells dying around the mutant clones; the Z projection confirms that dying cells are outside the lgl-/- tissue. Arrowheads indicate that dying cells are deprived of dIAP1. C: Surface and cross sections of a wild-type disc stained with Laminin A. C'-C"': Laminin A staining of lgl-/- clones in a M/+, lgl+/- background; arrows indicate several points in which basement membrane integrity is lost. D-E': lgl-/-; UAS-dmRNAi clones (GFP+) in a yw, hs-Flp, tub>Gal4, UAS-GFP/+;l(2)gl4, FRT40A/M(2)24F, tub>Gal80, FRT40A; UAS-dmRNAi/+ background (GFP-). D, D': aPKC staining reveals that lgl-/-; UAS-dmRNAi mutant cells in a M/+, lgl+/- background do not lose apical-basal polarity (compare with 6A). Heat shock pulses were 20 minutes (D) and 1 hour duration (D'). E, E': Caspase staining shows that apoptosis is either absent (E) or scattered throughout the disc (E', arrows). Scale bars are 35 μm. Mutant clone genotypes are indicated.