ΔTL
WT
| ATP | CTP | GTP | UTP |
|---|
ECA(cATP) | - | ~1500
20000
|
~4
~1200
|
~20
~3000
|
ECC(cCTP) | ~1000
~100000
| - | ~400
~30000
|
~20
~350
|
ECG2(cGTP) |
~40
~
450
| ~5000
~60000
| - |
~20
~750
|
ECU(cUTP) | ~2000
~100000
| ~500
~1000
| ~900
~65000
| - |
- Kinetic discrimination (folds) was quantified as a ratio of the rate of incorporation of 1 mM cATP, cCTP, cGTP and cUTP (measured in elongation complexes ECA, ECC, ECG1 and ECU, respectively (Additional File 1: Figure S2)) to the rate of misincorporation of these 10 mM nucleotide triphosphates (top row) in elongation complexes identified in the left column (Additional File 1: Figure S2). The data for ΔTL RNAP (plain) are approximate due to very low efficiency of some of the misincorporation events by ΔTL RNAP. The data for WT RNAP (Italic) are approximate because incorporation was fitted using single exponent equation.
