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Table 4 Discrimination against 2' and 3'-deoxy substrates by ΔTL RNA polymerase.

From: Stepwise mechanism for transcription fidelity

  cATP(ECA)/
c2'dATP(ECA)
cCTP(ECC)/
c2'dCTP(ECC)
cGTP(ECG3)/
c2'dGTP(ECG2)
cUTP(ECU)/
TTP(ECU)
cATP(ECA)/
c3'dATP(ECA)
cGTP(ECG3)/
c3'dGTP(ECG2)
Discrimination,
folds
~6 ~11 ~3 ~17 ~3 ~0.4
  1. Kinetic discrimination was quantified as a ratio of the rate of incorporation of 1 mM cNTP to the rate of misincorporation of 1 mM of a corresponding c2'dNTP or c3'dNTP in elongation complexes identified.