Immobilised Tol scaffold containing the osteopontin motif, SVVYGLR, specifically controls cell adhesion and cell morphology. Cells were plated onto surfaces displaying scaffold proteins in the absence of serum supplements and assessed after 24 hours. Immunolocalization of vinculin and counterstaining with DAPI allowed identification of focal adhesion complexes (white arrow) in cells on (a) Tol-OPN-ST; (b) Tol-GFP-ST; (c) Tol-BMP-ST; (d) TEG. Size bars = 200 μm and 50 μm in the inset images. Image analysis demonstrated significant differences in (e) the number of cells adhered to Tol-OPN-ST compared to all other surfaces; (f) the area of cells on Tol-OPN-ST compared to TEG; (g) the circularity of cells on Tol-OPN-ST compared to the other surfaces and (h) abundance of vinculin positivity in cells on Tol-OPN-ST compared to other surfaces. Data representative of three independent experiments; results are mean ± SEM; *P < 0.05, ** P < 0.01. Significance was determined by ANOVA with Fisher post test.