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Table 1 SPR data of the Tol, Tol-Osteopontin (Tol-OP) and Tol-BMP proteins binding to a gold surface in SPR analysis (BIAcore).

From: Controlled spatial and conformational display of immobilised bone morphogenetic protein-2 and osteopontin signalling motifs regulates osteoblast adhesion and differentiation in vitro

Protein

rU increase

Mr of protein

moles/mm2

Molecules/mm2

Tol

1,526.53

1,4874.4

1.03 × 10-13

6.4 × 1010

Tol-Op

4,381.15

18,407.3

2.38 × 10-13

14.8 × 1010

Tol-BMP

3,162.26

17,894.8

1.77 × 10-13

11.0 × 1010

  1. The proteins were added at a concentration of 5 mM in a total volume of 30 μl over a period of 15 minutes.
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BMC Biology

ISSN: 1741-7007

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