Table 1 Primers used for the generation of recombinant mouse monoclonal antibodies.
From: A rapid and scalable method for selecting recombinant mouse monoclonal antibodies
Primers used for the generation of a leader sequence for the κ light chains | |
|---|---|
1 | GCCACCATGGAGTTTCAGACCCAGGTACTCATGTCCCTGCTGCTCTGCATGTCTGGTGC |
2 | GGCCGCACCAGACATGCAGAGCAGCAGGGACATGAGTACCTGGGTCTGAAACTCCATGGTGGC |
Degenerate forward primers used for the amplification of rearranged κ light chains | |
3 | TCAATAGTTGAACATAGCGGCCGCASAAAWTGTKCTCACCCAGTC |
4 | TCAATAGTTGAACATAGCGGCCGCAGAWATTGTGCTMACTCAGTC |
5 | TCAATAGTTGAACATAGCGGCCGCAGACATTGTGCTRACACAGTC |
6 | TCAATAGTTGAACATAGCGGCCGCAGACATTGTGATGACMCAGTC |
7 | TCAATAGTTGAACATAGCGGCCGCAGAYATCMAGATRAMCCAGTC |
8 | TCAATAGTTGAACATAGCGGCCGCAGAYATCCAGATGAYTCAGTC |
9 | TCAATAGTTGAACATAGCGGCCGCAGATATCCAGATGACACAGAC |
10 | TCAATAGTTGAACATAGCGGCCGCAGACATTGTGCTGACCCAATC |
11 | TCAATAGTTGAACATAGCGGCCGCAGACATYSTRATGACCCARTC |
12 | TCAATAGTTGAACATAGCGGCCGCAGATRTTKTGATGACYCARAC |
13 | TCAATAGTTGAACATAGCGGCCGCAGAYATTGTGATGACBCAGKC |
14 | TCAATAGTTGAACATAGCGGCCGCAGATATTGTGATAACCCAGGA |
15 | TCAATAGTTGAACATAGCGGCCGCAGACATCYTGCTGACYCAGTC |
16 | TCAATAGTTGAACATAGCGGCCGCAGAAAWTGTGYTGACCCAGTC |
17 | TCAATAGTTGAACATAGCGGCCGCAGAAACAACTGTGACCCAGTC |
18 | TCAATAGTTGAACATAGCGGCCGCAGACATTRTGATGWCACAGTC |
19 | TCAATAGTTGAACATAGCGGCCGCAGACATCCAGMTGACMCARTC |
Reverse primer used for the amplification of rearranged κ light chains | |
20 | GGATACAGTTGGTGCAGCATCAGCCC |
Primers used for the generation of the joining fragment | |
21 | GGGCTGATGCTGCACCAACTGTA |
22 | TCAAGTGCAAAGACTCACTTTATTG |
23 | CAATAAAGTGAGTCTTTGCACTTGATAGTTATTAATAGTAATCAATTACG |
24 | TAGCAGAACAGGCAGCTTCATGGTGGCCTGGGAGTGGACACCTGTGGAGAG |
25 | ATGAAGCTGCCTGTTCTGCTAGTG |
26 | ACTGCTTGAGGCTGGACTCGTGAACAATAGCAGC |
Degenerate forward primers used for the amplification of rearranged heavy chains | |
27 | TTCACGAGTCCAGCCTCAAGCAGTGAKRTRCAGCTTMAGGAGTC |
28 | TTCACGAGTCCAGCCTCAAGCAGTGAGKTYCAGCTBCAGCAGTC |
29 | TTCACGAGTCCAGCCTCAAGCAGTCAGGTGCAGMTGAAGSAGTC |
30 | TTCACGAGTCCAGCCTCAAGCAGTGAGRTCCAGCTGCAACARTC |
31 | TTCACGAGTCCAGCCTCAAGCAGTCAGGTYVAGCTGCAGCAGTC |
32 | TTCACGAGTCCAGCCTCAAGCAGTCAGGTYCARCTGCAGCAGTC |
33 | TTCACGAGTCCAGCCTCAAGCAGTGAGGTGMAGCTGGTGGAATC |
34 | TTCACGAGTCCAGCCTCAAGCAGTGAVGTGMWGCTSGTGGAGTC |
35 | TTCACGAGTCCAGCCTCAAGCAGTGARGTGCAGCTGKTGGAGWC |
36 | TTCACGAGTCCAGCCTCAAGCAGTGAGGTGAAGCTGATGGAATC |
37 | TTCACGAGTCCAGCCTCAAGCAGTGAGGTGCAGCTTGTTGAGTC |
38 | TTCACGAGTCCAGCCTCAAGCAGTGAGGTGAAGCTTCTCRAGTC |
39 | TTCACGAGTCCAGCCTCAAGCAGTGAAGTGAARMTTGAGGAGTC |
40 | TTCACGAGTCCAGCCTCAAGCAGTCAGGTTACTCWGAAAGWGTCTG |
41 | TTCACGAGTCCAGCCTCAAGCAGTCAGGTCCAACTGCAGCAGCC |
42 | TTCACGAGTCCAGCCTCAAGCAGTGATGTGAACCTGGAAGTGTC |
43 | TTCACGAGTCCAGCCTCAAGCAGTCAGATCCAGTTSGTRCAGTC |
44 | TTCACGAGTCCAGCCTCAAGCAGTGAGGTRCAGCTKGTAGAGAC |
Reverse primers used for the amplification of rearranged heavy chains | |
45 | CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACCGTGGTCC |
46 | CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACTGTGAGAGTGGTGC |
47 | CTATTCTAGCTAATCTAGGCGCGCCGCAGAGACAGTGACCAGAGTCC |
48 | CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACTGAGGTTC |
Primers used for the amplification of the IgG1 constant region | |
49 | ATATCACGGCGCGCCCGACACCCCCATCTGTCTATCCA |
50 | GACTGATCTAGATCATTTACCAGGAGAGTGGGAGAG |
Primers used for fusion polymerase chain reaction | |
51 | TCAATAGTTGAACATAGCGGCCGC |
52 | CTATTCTAGCTAATCTAGGCGCGCCG |
Primer specific for the aberrant κ light chain from SP2/0 myeloma | |
53 | GGATGCTGCAACCTATTACTGTCAGCACATTAGGGAGCTTACACG |