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Table 1 Yeast strains and plasmids

From: Sumo-dependent substrate targeting of the SUMO protease Ulp1

Name Pertinent genotypes or parent strain Plasmids Study/source
MHY500 Matα his3-Δ200 leu2-3, 112 ura3-52 lys2-801trp1-1
gal2
  Li and Hochstrasser, 2003 [26]
BY4743 MATa leu2Δ0 met15Δ0 ura3Δ0   Open Biosystems
(Huntsville, AL, USA)
YOK 1611 MHY500 ULP1-GFP/LEU2 This study
YOK 1474 ULP1 (C580S) -GFP/LEU2
YOK 1490 ULP1(Reg1)-GFP/LEU2
YOK 1861 UlLP1(Reg2)-GFP/LEU2
YOK 1479 ULP1(Δ2)-GFP/LEU2
YOK 2016 ULP1 (D451N C580S) -GFP/LEU2
YOK 1839 ULP1(Reg3)-GFP/LEU2
YOK 1907 ULP1(Reg3 (C580S) )-GFP/LEU2
YOK 1903 ULP1((Reg3ΔSBS)-GFP/LEU2
YOK 2203 ULP1(SBS)-GFP/LEU2
YOK 1828 ULP1((Reg3 (ts) )-GFP/LEU2
YOK 2157 ULP1((Reg3 (ts C580S) )-GFP/LEU2
YOK 1857 SMT3-GFP/LEU2 Panse et al., 2003 [25]
YOK 44 smt3-331   Biggins et al., 2001 [45]
YOK 1995 ULP1 (C580S) -GFP/LEU2 This study
YOK 847 ubc9-1   Betting and Seufert, 1996 [44]
YOK 2065 ULP1 (C580S) -GFP/URA3 This study
YOK 2144 SMT3-GFP/URA3
GBY1 MAT a smt3 R11, 15, 19::TRP1   Bylebyl et al., 2003 [13]
YOK 1910 GBY1 ULP1 (C580S) -GFP/LEU2 This study
yDS880 MAT a -inc ade2-101 his3-200 leu2-1::GAL-HO-LEU2 lys2-801 RAD53::FLAG-HIS3 siz1::NAT
siz2::HPH sml1::KAN trp1-63 ura3-52 VII-L::TRP-HO site-LYS2
  Schwartz et al., 2007 [46]
YOK 2067 ULP1 (C580S) -GFP/URA3 This study
YOK 2143 SMT3-GFP/URA3
kap121ts kap121::ura3::HIS3 ura3-52 his3Δ200 trp1-1 leu2-3, 112 lys2-801 pRS314-kap121-34 Leslie et al., 2002 [48]
YOK 1487 kap121ts ULP1-GFP/LEU2 This study
YOK 1488 kap121ts ULP1 (C580S) -GFP/LEU2
YOK 1944 kap121ts ULP1(Reg3 (C580S) )-GFP/LEU2
AH109 MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UASMEL1TATA-lacZ, MEL1   (cat. no. 630444; Clontech, Mountain View, CA, USA)
YOK 2212 AH109 ULP1 (Reg3 (D451N C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1 This study
YOK 2173 ULP1 (Reg3 (C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1
YOK 2175 ULP1 (Reg3 (D451N) )-pOAD/LEU2 + SMT3-pOBD/TRP1
YOK 2177 ULP1 (Reg3 (ts) )-pOAD/LEU2 + SMT3-pOBD/TRP1
YOK 2181 ULP1 (Reg3 (ts C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1
YOK 2183 SMT3-pOAD/LEU2 + SLX5 pOBD/TRP1
YOK 2185 vector-pOAD/LEU2 + vector pOBD/TRP1
YOK 428 ulp1::KAN (segregant of heterozygous diploid ULP1/ulp1::KAN in BY4743 - (Open Biosystems, Huntsville, AL, USA (cat. no. YSC1021-671376) ulp1ts/TRP/NAT
GPD-FLAG-SMT3gg/pRS425