Table 1 Yeast strains and plasmids
From: Sumo-dependent substrate targeting of the SUMO protease Ulp1
Name | Pertinent genotypes or parent strain | Plasmids | Study/source |
|---|---|---|---|
MHY500 | Matα his3-Δ200 leu2-3, 112 ura3-52 lys2-801trp1-1 gal2 |  | Li and Hochstrasser, 2003 [26] |
BY4743 | MATa leu2Δ0 met15Δ0 ura3Δ0 |  | Open Biosystems (Huntsville, AL, USA) |
YOK 1611 | MHY500 | ULP1-GFP/LEU2 | This study |
YOK 1474 | ″ | ULP1 (C580S) -GFP/LEU2 | ″ |
YOK 1490 | ″ | ULP1(Reg1)-GFP/LEU2 | ″ |
YOK 1861 | ″ | UlLP1(Reg2)-GFP/LEU2 | ″ |
YOK 1479 | ″ | ULP1(Δ2)-GFP/LEU2 | ″ |
YOK 2016 | ″ | ULP1 (D451N C580S) -GFP/LEU2 | ″ |
YOK 1839 | ″ | ULP1(Reg3)-GFP/LEU2 | ″ |
YOK 1907 | ″ | ULP1(Reg3 (C580S) )-GFP/LEU2 | ″ |
YOK 1903 | ″ | ULP1((Reg3ΔSBS)-GFP/LEU2 | ″ |
YOK 2203 | ″ | ULP1(SBS)-GFP/LEU2 | ″ |
YOK 1828 | ″ | ULP1((Reg3 (ts) )-GFP/LEU2 | ″ |
YOK 2157 | ″ | ULP1((Reg3 (ts C580S) )-GFP/LEU2 | ″ |
YOK 1857 | ″ | SMT3-GFP/LEU2 | Panse et al., 2003 [25] |
YOK 44 | smt3-331 | Â | Biggins et al., 2001 [45] |
YOK 1995 | ″ | ULP1 (C580S) -GFP/LEU2 | This study |
YOK 847 | ubc9-1 | Â | Betting and Seufert, 1996 [44] |
YOK 2065 | ″ | ULP1 (C580S) -GFP/URA3 | This study |
YOK 2144 | ″ | SMT3-GFP/URA3 | ″ |
GBY1 | MAT a smt3 R11, 15, 19::TRP1 | Â | Bylebyl et al., 2003 [13] |
YOK 1910 | GBY1 | ULP1 (C580S) -GFP/LEU2 | This study |
yDS880 | MAT a -inc ade2-101 his3-200 leu2-1::GAL-HO-LEU2 lys2-801 RAD53::FLAG-HIS3 siz1::NAT siz2::HPH sml1::KAN trp1-63 ura3-52 VII-L::TRP-HO site-LYS2 | Â | Schwartz et al., 2007 [46] |
YOK 2067 | ″ | ULP1 (C580S) -GFP/URA3 | This study |
YOK 2143 | ″ | SMT3-GFP/URA3 | ″ |
kap121ts | kap121::ura3::HIS3 ura3-52 his3Δ200 trp1-1 leu2-3, 112 lys2-801 | pRS314-kap121-34 | Leslie et al., 2002 [48] |
YOK 1487 | kap121ts | ULP1-GFP/LEU2 | This study |
YOK 1488 | kap121ts | ULP1 (C580S) -GFP/LEU2 | ″ |
YOK 1944 | kap121ts | ULP1(Reg3 (C580S) )-GFP/LEU2 | ″ |
AH109 | MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UASMEL1TATA-lacZ, MEL1 |  | (cat. no. 630444; Clontech, Mountain View, CA, USA) |
YOK 2212 | AH109 | ULP1 (Reg3 (D451N C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1 | This study |
YOK 2173 | ″ | ULP1 (Reg3 (C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1 | ″ |
YOK 2175 | ″ | ULP1 (Reg3 (D451N) )-pOAD/LEU2 + SMT3-pOBD/TRP1 | ″ |
YOK 2177 | ″ | ULP1 (Reg3 (ts) )-pOAD/LEU2 + SMT3-pOBD/TRP1 | ″ |
YOK 2181 | ″ | ULP1 (Reg3 (ts C580S) )-pOAD/LEU2 + SMT3-pOBD/TRP1 | ″ |
YOK 2183 | ″ | SMT3-pOAD/LEU2 + SLX5 pOBD/TRP1 | ″ |
YOK 2185 | ″ | vector-pOAD/LEU2 + vector pOBD/TRP1 | ″ |
YOK 428 | ulp1::KAN (segregant of heterozygous diploid ULP1/ulp1::KAN in BY4743 - (Open Biosystems, Huntsville, AL, USA (cat. no. YSC1021-671376) | ulp1ts/TRP/NAT GPD-FLAG-SMT3gg/pRS425 | ″ |