Jhd2 regulates unequal sister chromatid exchange in rDNA in a Sir2- independent manner. (A, C) The frequency of unequal rDNA crossovers was monitored by loss of the ADE2 gene located within the rDNA array for WT (W303R) and the indicated deletion strains. (B, D) The percentage of ADE2 gene loss (% marker loss) from (A) and (C) was calculated as the ratio of red-sectored colonies to the total number of colonies. Completely red colonies were excluded. Error bars indicate the SD from two (B) or three (D) repetitions, and asterisks indicate statistically significant differences compared with WT or between indicated pairs (*, P <0.05; **, P <0.01). WT, wild type.