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Figure 1 | BMC Biology

Figure 1

From: Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

Figure 1

Summary of 16S rRNA gene sequencing taxonomic assignment from ten-fold diluted pure cultures and controls. Undiluted DNA extractions contained approximately 108 cells, and controls (annotated in the Figure with 'con') were template-free PCRs. DNA was extracted at ICL, UB and WTSI laboratories and amplified with 40 PCR cycles. Each column represents a single sample; sections (a) and (b) describe the same samples at different taxonomic levels. a) Proportion of S. bongori sequence reads in black. The proportional abundance of non-Salmonella reads at the Class level is indicated by other colours. As the sample becomes more dilute, the proportion of the sequenced bacterial amplicons from the cultured microorganism decreases and contaminants become more dominant. b) Abundance of genera which make up >0.5% of the results from at least one laboratory, excluding S. bongori. The profiles of the non-Salmonella reads within each laboratory/kit batch are consistent but differ between sites.

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