5'-Extension of TRM12 mRNA leads to reduced Trm12 protein levels. A) IGV profiles showing apparent approximately 800 base extension of TRM12 mRNA in tfc6 mutant. B) Extended TRM12 5'-UTR levels are relatively low in tfc6 mutants, but much more abundant in tS(AGA)M B-box mutants DDY5162 and DDY5163. Extended RNAs were detected by RT-PCR as in previous figures. C) Western blot of TRM12-9 × -myc strains shows reduced Trm12 protein levels in the B-box mutants. TRM12-9 × -myc tagged strains are: wild type, DDY5170 and DDY5171; tfc6, DDY5172 and DDY5173; and ts(aga)m b-box, DDY5174, DDY5205 and DDY5206. IGV, integrative genomics viewer.