Histology of neonatal and adult livers stained with Oil Red O. A) An increased number of lipid droplets was observed in cryosectioned Grb10 m/+ and Grb10 m/+/Dlk1 +/p neonatal livers following Oil Red O staining. B) An increased number of lipid droplets was observed in Oil Red O stained adult livers from Dlk1 +/p knockout mice. Images presented in Panels A and B show representative sections for each of the analysed genotypes. C) Quantification of histological sections (from three individuals for each genotype) of neonatal liver stained with Oil red O (percentage of area stained), showing increased staining for both Grb10 m/+ and Grb10 m/+/Dlk1 +/p livers. D) Table summarising results of statistical analysis of data in C. All values represent means ± SEM and have been subject to one way ANOVA with post hoc Tukey’s analysis. WT n = 3, Dlk1 +/p n = 3, Grb10 m/+ n = 3 and Grb10 m/+/Dlk1 +/p n = 3; ** P <0.01, ***P <0.001. ANOVA, analysis of variance; SEM, standard error of the mean; WT, wild type.