Figure 2

NCD4lo cells show higher CD5 levels but no difference in proximal signaling. A. Ex vivo analysis of stained splenic cells to show gated NCD4lo and NCD4hi cells. B. Comparison of CD5 levels as log (left) and linear (right) plots between NCD4hi and NCD4lo cells. C. CD5 MFI values for NCD4hi and NCD4lo cells (Mean ± SE, one of three experiments). D. Comparison of forward scatter between NCD4hi and NCD4lo cells. E. Forward scatter values on NCD4hi and NCD4lo cells (Mean ± SE, one of five experiments). F. Comparison of TCRβ levels as log (left) and linear (right) plots between NCD4hi and NCD4lo cells. G. TCRβ MFI values for NCD4hi and NCD4lo cells (Mean ± SE, one of three experiments). H. Representative profile of Fluo-3/Fura-Red ratio in NCD4hi and NCD4lo cells at rest and post-activation. I. Pooled data from independent mouse spleens showing increase in Fluo-3/Fura-Red ratio with anti-CD3 (10 μg/ml) + anti-CD28 (3 μg/ml) treatment J. Pooled data for fold increase over background in pZap-70 staining MFI in NCD4hi and NCD4lo cells activated with anti-CD3 + anti-CD28, mean ± SE. MFI, mean fluorescence intensity; NCD4, naïve CD4 T cells; SE, standard error; TCR, T cell receptor.