Figure 4

NCD4lo and NCD4hi cells from human PBMCs show properties similar to mouse cells. A. A representative gating strategy for NCD4lo and NCD4hi cells (left), histogram overlay of sorted NCD4lo and NCD4hi cells (middle) and analysis of the same cells for size (right). B. Forward scatter for NCD4hi and NCD4lo cells from individual donors (Mean ± SE). C. 3H-Thymidine incorporation in response to titrating doses of anti-CD3 and 3 μg/ml of anti-CD28 60 hours post-activation (Mean + SE of triplicate cultures; one of four independent donors). D. Histogram overlays for MG staining in log (left) and linear (right) scale in NCD4lo and NCD4hi. E. MFI for MG on NCD4hi and NCD4lo cells from six individual donors (mean ± SE). F. Histogram overlays for MR staining in log (left) and linear (right) scale in NCD4lo and NCD4hi. G. MFI for MR on NCD4hi and NCD4lo cells from six individual donors (mean ± SE). MFI, mean fluorescence intensity; MG, Mitotracker Green; MR, Mitotracker Red; NCD4, naïve CD4 T cells; PBMC, peripheral blood mononuclear cells; SE, standard error.