Figure 6

Consequences of prolonged tonic signaling and absence of tonic signaling in naïve T cells. A. Numbers of adoptively transferred NCD4 OT-II cells recovered from spleens three days after transfer into WT and MHCII-null mice (one of three independent experiments). B. Comparison of CD4 levels (plotted on log on left and linear scale on right) of donor NCD4 OT-II cells after three days in WT and MHCII-null mice. C. Pooled data, of paired samples showing CD4 MFI and mean ± SE (one of three experiments). D. Comparison of CD5 levels (plotted on log on left and linear scale on right) of donor NCD4 OT-II cells after three days in WT and MHCII-null mice. E. Pooled data, of paired samples showing CD5 MFI and mean ± SE (one of three experiments). F. Proliferation assay of donor NCD4 OT-II cells from WT or MHCII-null recipient mice four days post-transfer in response to OVA-II peptide in the presence of irradiated DCs from WT mice. Data were normalized for 1,000 donor NCD4 OT-II cells (Mean ± SE of triplicate cultures; data representative of two experiments; three recipient mice per group per experiment). G. Representative DCFDA staining (plotted on log on left and linear scale on right) for donor OT-II cells from WT or MHCII-null spleen cells seven days post-transfer. H. Pooled data, of paired samples showing DCFDA MFI values (Mean ± SE, one of three experiments) in WT or MHCII-null recipients seven days after transfer. DCs, dendritic cells; DCFDA, 2-7-dichlorofluorescin diacetate; MFI, mean fluorescence intensity; NCD4, naïve CD4 T cells; SE, standard error; WT, wild type.