Figure 3
From: The toxoplasma-host cell junction is anchored to the cell cortex to sustain parasite invasive force
RON2-expressing tachyzoites enter through a stable (A, B) or capped (C, D) junction. DICmCherry (green) time lapses showing the penetration of tachyzoites into (A) HFF (top panel), HeLa (bottom panel), (B) Myr-Palm-GFP- HeLa cells (red), (C) Ptk-1 (top panel), HFF (bottom panel), (D) MyrPalm-GFP- HeLa (red); the blue and pink lines depict the trajectories of the parasite apex and the RON2- marked junction (green), respectively; pink arrowheads define the first and last signs of the junction; graphs on the right show the tachyzoite (blue) and the junction (pink) speeds over time; the PM movement during cell penetration is tracked with the yellow lines (B, D); all scale bars: 5Â ÎĽm. DIC, HeLa, human epithelial cervical cancer cells; HFF, human foreskin fibroblasts; Myr-PALM, myristoylated and palmitoylated; PM, plasma membrane; Ptk-1, rat kangaroo kidney epithelial cells; PV, parasitophorous vacuole; RON2, RhOptry Neck.