Figure 6

Δ toxofilin parasites form more capped junctions than toxofilin ⁺parasites. (A) Pie graphs showing the distribution of stable (blue), capped (orange), end-capped junctions (red) and failed (yellow) events in epithelial and fibroblastic cells, the absolute numbers are indicated; note the increase in invasion failure when tachyzoites lack toxofilin and in capped junctions when they infect epithelial cells; (B) Scatter graphs showing the speed as a function of time for toxofilin⁺ RON2mC (dark dots) or Δ toxofilin RON2mC (pale dots) parasites and for stable (blue) or capped (pink) junctions; note the significantly faster process when the toxofilin⁺ tachyzoites enter with static junctions; (C) DIC-Cherry (green) merged time lapse showing the capping of the RON2-labeled junction during entry of a Δ toxofilin RON2mC parasite in a HFF cell; (D) DIC mC (green) merged time lapse showing a Δ toxofilin RON2mC parasite penetrating into a GFP-PH-PLCδ HeLa cell (red), the pink arrowhead shows the time when the parasite is enclosed in the host cell PM; (E) DIC-mCherry (green) merged time lapse showing Δ toxofilin RON2mC entering in a Myr-Palm-GFP expressing HeLa cell (red); pink arrowheads define the first and last signs of the junction; trajectories of: the parasite apex (blue line), the junction (pink line) and the PM (yellow line) are shown; graphs on the right show the tachyzoite (blue), the junction (pink) and the host cell PM (yellow) speeds over time; all scale bars: 5 μm; note that in (E) the tachyzoite creates a host cell PM evagination when moving forward and then translocates the host cell PM at the time it stops progressing, all scale bars: 5 μm. DIC, HeLa, human epithelial cervical cancer cells; HFF, human foreskin fibroblasts; Myr-Palm, myristoylated and palmitoylated; PM, plasma membrane; RON2, RhOptry Neck.