Relative temperature stability of dADAR isoforms. (A) Western blot analysis of HA-tagged hardwired S and G dADAR isoforms . The hypomorphic allele at 20°C is presented for comparison. β-actin is presented as a loading control. Wild type dADAR, which lacks the HA tag is presented as a negative control (−HA). (B) Quantification of western blot analysis. dADAR-HA signal from S (white) and G (black) alleles is normalized to the β-actin signal from each lane. Bars represent standard deviation.