T.C.RNAi provides detailed profiles of LET-60Ras in RMD neurons. (A) Representative images of GLR-1::GFP in neurites of RMDD of transgenic animals with let-60 time- and RMD-specific RNAi (T.C.RNAi) (rectangle = the neurite of RMDD; yellow arrowheads = GLR-1::GFP clusters). (B) Average intensity of GLR-1::GFP fluorescence in RMDD neurons of transgenic animals with let-60 T.C.RNAi after heat shock at the embryonic or adult stage (n ≥10 animals). (C) The proportion of wild type, let-60(lf) mutants, and animals with let-60 T.C.RNAi after heat shock treatment exhibiting CL (n ≥3 assays). In (B) and (C), RMD::(s) and hsp::(as) mean rig-5ap::let-60(s) and hsp16-2::let-60(as), respectively. Error bars represent SEM and asterisks indicate significant differences (*P <0.05, **P <0.01, ***P <0.001, Dunnett’s test or Student’s t-test). SEM, standard error of the mean.