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Figure 1 | BMC Biology

Figure 1

From: The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers

Figure 1

DNA methylation is increased at ~ one thousand loci in the genomes of Rlf MommeD28/MommeD28 mice. (A) A UCSC Genome Browser snapshot showing a representative Rlf-DMR, within the gene Bai2, with increased methylation in Rlf MommeD28/MommeD28 compared to Rlf +/+ mice. The gene’s exons are indicated by blue boxes, introns by connecting lines and direction of transcription by the intronic arrows. (B) The mice carry a GFP transgene used in the ENU mutagenesis screen that identified Rlf as an epigenetic modifier [1]. Bisulphite sequencing reads covering the transgene show hypermethylation of DNA from Rlf MommeD28/MommeD28 mice throughout its length. Transgene sequence features, and a region previously targeted by bisulphite PCR [1], are indicated. (C) The average methylation of regions differentially methylated in Rlf MommeD28/MommeD28 mice, compared to wild-type, are plotted. Only Rlf-DMRs >2.5 kb from TSS were included (n = 1,074). (D) Average PhastCons scores for placental mammals at Rlf-DMRs. (E) Scatterplot of average CpG methylation observed in Rlf MommeD28/MommeD28 mutants compared to wild-types for 1,329 Rlf-DMRs identified in E14.5 liver.

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BMC Biology

ISSN: 1741-7007

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