Figure 5

Biological effects of Uhg genes. A) Uhg1 locus with adjacent genes. Black arrows indicate direction of transcription, blue rectangles and lines correspond to exons and introns, respectively, red triangles show snoRNAs. Below are indicated the genomic regions used for the luciferase reporter (with the single E-box shown as a vertical line) and the extent of the deletion in the Uhg1 ¹ null allele. B) Amino acid incorporation rates in wandering larvae. Ratios (Uhg1 ¹/Uhg1 ^rev) and standard deviations are shown for three biological replicates each with ten larvae for each genotype. Statistical significance of the difference P = 0.01 (Student’s two-tailed t-test). C) Average time from egg deposition to adult eclosion for male flies; numbers of analyzed flies are indicated in parentheses. ‘mut’ corresponds to ‘Uhg1 ¹’, ‘rev’ to ‘Uhg1 ^rev’, ‘+’ to the standard lab strain ‘y w’. D) Average area of salivary gland nuclei overexpressing Myc (‘Myc OE’) relative to neighboring nuclei without Myc overexpression (‘ctr’). Each bar represents 39 to 52 nuclei from 10 separate salivary glands; error bars indicate SEM. E) Luciferase assays from single adult males overexpressing the indicated Uhg-transgenes under brat-knockdown (brat-kd) or brat-wildtype (ctr) conditions in type II neuroblast lineages. #1 and #2 correspond to independent transgenes; numbers in parentheses indicate the numbers of individually assayed flies (originating from two to ten separate experiments). Genotypes: in addition to the indicated UAS-Uhg transgene, the flies carried ‘worniu-GAL4 asense-GAL80/+; UAS-brat-(inverted repeat) UAS-Luciferase/+’. Difference to control is <0.05 (*) and <0.005 (**), respectively (Student’s two-tailed t-test). SEM, standard error of the mean; snoRNA, small nucleolar RNA.