Transcript accumulation of selected JA-responsive and ET-related genes in stamens of JA-treated flowers buds. Flower buds in stage 2 were treated three times in an interval of 20 hours by spraying with JA solution in the concentration indicated. Stamens were harvested from flower buds at stage 5 (five hours after the last treatment). (a) Relative transcript levels of genes encoding early JA-induced genes in stamen of wild type plants. (b) Relative transcript levels of genes encoding ET biosynthetic enzymes, proteins involved in ET-signaling, and of ET-response genes in stamen of AOC-RNAi plants. All transcript levels were determined by RT-qPCR and set in relation to SlTIP41. Mean values + SD are shown. Data of JA treated stamen were compared with stamen of control treatment (0 μM JA) by Student’s t-test (*P ≤0.05, **P ≤0.01, n = 4). ET, ethylene; JA, jasmonic acid; SD, standard deviation.