Fig. 1

Growth trajectories of wild-type and black mutant fifth instar Manduca larvae fed rapamycin or DMSO. a, b The growth trajectories of the wild-type (a) and the black mutant (b) larvae. The solid line with closed circles is the control animals and the dashed line with open circles is the rapamycin-treated animals. Blue lines represent critical weights as determined in Fig. 3. c, d The average peak masses (c) and growth rates (d) of the two strains raised on different diet treatments. Gray bars represent animals reared on diets with rapamycin, and black bars represent those reared on diets with DMSO. Bars with different letters represent statistically significant differences (one-way ANOVA, P < 0.001 for the peak masses and P < 0.0001 for growth rates). Error bars represent standard errors