Fig. 6

Epigenetic profile of the X chromosome during the late stages of the male germline. a Binding peaks of histone marks on PS/RS inactive Timp1 gene locus in GS, PS, and RS. b Binding peaks of histone marks on RS active Akap4 gene locus in GS, PS, and RS. ATD of histone marks in representative groups in c GS, d PS, and e RS. For H3K4me2, ATD on gene bodies from TSS to TES and ±5 kb regions were analyzed. f Average RPKM of each group in GS, PS, RS, and ES. g, h ATD profiles of H3K4me2 on gene bodies and Kcr on TSS are compared between WT and Rnf8 KO. Wilcoxon rank sum test was performed for read counts in the highlighted area (H3K4me2: −1 kb from TSS to TES; Kcr: −500 bp to +500 bp from TSS, *P <0.05, **P <0.001). ATD, average tag density; ES, embryonic stem cells; GS, germline stem cells; Kcr, lysine crotonylation; KO, knockout; PS, pachytene spermatocytes; RS, round spermatids; RPKM, reads per kilobase per million; TES, transcription end site; TSS, transcription start site; WT, wild-type