Skip to main content

Advertisement

Fig. 6 | BMC Biology

Fig. 6

From: Phosphoproteome dynamics mediate revival of bacterial spores

Fig. 6

The phosphorylation state of HPr, the master regulator of carbon metabolism, is crucial for spore revival. a Spores of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains were incubated at 37 °C in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and optical density (OD600) was measured at the indicated time points. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. b Growth curves of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains. Cells were grown at 37 °C in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and OD600 was measured at the indicated time points. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. c Spores of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains were incubated in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and monitored by time lapse microscopy. Shown are phase-contrast images acquired at the indicated time points. Arrows signify the dominating revival stages of each population at a given time point (germination, red; ripening, orange; elongation, green). Scale bar represents 1 μm

Back to article page