Fig. 6

The phosphorylation state of HPr, the master regulator of carbon metabolism, is crucial for spore revival. a Spores of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains were incubated at 37 °C in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and optical density (OD₆₀₀) was measured at the indicated time points. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. b Growth curves of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains. Cells were grown at 37 °C in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and OD₆₀₀ was measured at the indicated time points. The data points are averages of results obtained from three independent biological repeats. Error bars designate SD. c Spores of PY79 (wild type, WT), AR213 (HPr-S46A), AR214 (HPr-S46D), AR88 (∆crh), AR129 (HPr-S46A, ∆crh), AR130 (HPr-S46D, ∆crh), AR127 (∆HPr), AR128 (∆HPr, ∆crh), and AR196 (∆hprK) strains were incubated in S7-defined medium supplemented with L-Ala (10 mM) and glucose as a sole carbon source, and monitored by time lapse microscopy. Shown are phase-contrast images acquired at the indicated time points. Arrows signify the dominating revival stages of each population at a given time point (germination, red; ripening, orange; elongation, green). Scale bar represents 1 μm