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Table 4 Composition of protein bound and free intracellular amino acids of P. pastoris grown on glucose or methanol/glycerol in chemostats at μ = 0.1 h−1

From: Systems-level organization of yeast methylotrophic lifestyle

  Protein bound amino acids Free intracellular amino acids
  Methanol/glycerol Glucose Log2FC Methanol/glycerol Glucose Log2FC P value
  Average [mg/gCDW] Average [mg/gCDW] Average [mg/gCDW] Average [mg/gCDW]
Asx 48.8 30.2 0.69 5.42 2.91 0.89 0.00
Ala 32.1 20.2 0.67 0.90 1.02 −0.18 0.28
Arg 29.6 25.9 0.19 11.1 12.1 −0.12 0.47
Cys 5.59 4.56 0.30
Glx 87.0 68.0 0.36 22.3 29.4 −0.40 0.00
Gly 18.7 11.5 0.70
His 11.9 7.33 0.70 0.76 0.74 0.03 0.78
Ile 17.8 10.6 0.76 0.05 0.04 0.28 0.00
Leu 36.0 22.6 0.67 0.10 0.08 0.35 0.00
Lys 37.5 24.5 0.62 0.76 0.92 −0.28 0.18
Met 5.34 4.33 0.30 0.04 1.08 −4.63 0.00
Phe 20.0 12.9 0.63 0.03 0.03 0.03 0.56
Pro 0.92 2.76 −1.58 0.00
Ser 25.9 19.0 0.44 0.25 0.52 −1.04 0.00
Thr 28.2 19.3 0.55 0.24 0.27 −0.14 0.42
Tyr 17.3 8.89 0.96 0.06 0.05 0.18 0.02
Val 26.4 15.7 0.75 0.25 0.14 0.83 0.00
Total amino acids [mg/g Cell dry weight (CDW)] 448 306 0.55 43.2 52.1 −0.27 0.03
Total protein content [mg/gCDW] 540 390 0.47