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Table 3 Effect of hypoxia and hyperoxia selection on recombination rates in 16 regions of the D. melanogaster genome

From: Experimental evolution of recombination and crossover interference in Drosophila caused by directional selection for stress-related traits

  Control Hypoxia Control vs. Hypoxia Hyperoxia Control vs. Hyperoxia
Interval rf (%) χ 2a rf (%) χ 2a δ rf (%)b χ 2 c P d rf (%) χ 2a δ rf (%)b χ 2c P d
X chromosome
y-cv 13.15 ± 0.71 0.10 17.46 ± 0.80 6.31e 32.78 16.21 4.6 × 10–4 15.97 ± 0.77 2.37 21.44 7.21 4.1 × 10–2
cv-v 18.96 ± 0.83 0.57 26.29 ± 0.93 3.40 38.66 34.77 5.1 × 10–8 27.25 ± 0.94 1.99 43.72 43.43 2.1 × 10–9
v-f 22.57 ± 0.88 0.14 28.98 ± 0.96 2.04 28.40 24.30 9.8 × 10–6 27.52 ± 0.94 1.94 21.93 14.69 9.6 × 10–4
2 L chromosome
net-dp 12.20 ± 0.69 3.41 17.01 ± 0.79 1.79 39.43 21.07 4.2 × 10–5 14.50 ± 0.74 0.91 18.85 5.19 0.12
dp-b 30.48 ± 0.97 0.42 37.20 ± 1.02 0.08 22.85 22.05 2.8 × 10–5 36.16 ± 1.01 1.04 18.64 16.42 4.5 × 10–4
b-pk 6.65 ± 0.53 2.63 7.99 ± 0.57 0.14 20.15 2.98 0.38 6.65 ± 0.52 2.73 0.00 0.00 1
pk-cn 2.19 ± 0.31 0.71 2.12 ± 0.30 0.41 −3.20 0.03 1 1.95 ± 0.29 2.20 −10.96 0.32 1
2R chromosome
cn-kn 9.99 ± 0.63 0.37 10.38 ± 0.64 0.46 3.90 0.29 1 10.01 ± 0.63 0.89 0.20 0.02 1
kn-c 2.02 ± 0.30 1.69 1.73 ± 0.27 3.84 14.36 0.52 1 2.40 ± 0.32 1.02 18.81 0.77 1
c-px 24.67 ± 0.91 5.88 22.21 ± 0.88 0.54 −9.97 3.79 0.26 23.42 ± 0.89 3.76 −5.07 0.95 1
px-sp 7.58 ± 0.56 0.51 7.01 ± 0.54 2.73 −7.52 0.55 1 7.71 ± 0.56 1.82 1.72 0.03 1
3rd chromosome
ru-h 14.89 ± 0.75 2.94 14.84 ± 0.75 2.89 −0.34 0.01 1 14.39 ± 0.74 0.40 −3.36 0.22 1
h-th 11.82 ± 0.68 5.27 13.09 ± 0.71 3.34 10.74 1.67 0.80 11.66 ± 0.68 0.79 −1.35 0.03 1
th-cu 5.24 ± 0.47 4.52 6.58 ± 0.52 4.72 25.57 3.59 0.28 5.48 ± 0.48 1.82 4.58 0.13 1
cu-sr 5.98 ± 0.50 1.69 8.79 ± 0.60 1.40 46.99 13.08 1.8 × 10–3 6.70 ± 0.53 0.38 12.04 1.23 0.96
sr-e 4.50 ± 0.44 0.84 7.06 ± 0.54 0.28 56.89 13.55 1.6 × 10–3 4.75 ± 0.45 0.14 5.56 0.16 1
  1. For each cross, we scored 750 individuals of each line (3 lines each in control, hypoxia and hyperoxia variant) i.e., 6750 per cross. Thus, a total 27,000 flies were scored for recombination analysis in X chromosome, 2L arm, 2R arm and 3 chromosome in hypxoxia-hyperoxia experiment.
  2. a χ 2test for between-lines heterogeneity within either control of selection variants (df = 2)
  3. bδ rf (%), relative change of rf in selection variant compared to control variant
  4. c χ 2 test for significance between rf values in selection versus control (df = 1), see Methods
  5. dFalse discovery rate corrected P values (two-tailed test) based on χ 2c are present to take into account the effect of multiple comparisons
  6. e P <0.05; for more details of this table, see Methods and Additional file 3