Fig. 5
Nitrate enhances the expression of fatty acid oxidation enzymes and other mitochondrial proteins via NO-cGMP-PKG in C2C12 myotubes. (a) Cpt1b, Acad1 and Hadh, and (b) Ucp3, Cycs and Ndufs1 expression in C2C12 myotubes cultured and differentiated over 6 days in the presence of 0, 50 and 500 μM nitrate, (c and d) in the presence and absence of sGCi (ODQ, 1 μM), and (e and f) in the presence and absence of PKGi (KT5823, 1 μM). Data are represented as mean ± SEM, n = 4 repeats per condition. * P ≤0.05; ** P ≤0.01; *** P ≤0.001