Fig. 12

Subcellular compartmentalization of PS1, APP, and BACE1 in the presence and absence of Syt1. a Western blots show distribution of PS1, APP, and BACE1 in 13 subcellular fractions from parental and Syt1 KD cells. The arrows indicate a shift in the subcellular distribution in Syt1 KD cells. The enrichment of the respective subcellular compartments was determined by western blotting with anti-calreticulin, anti-GM130, anti-Tgn46, and anti-Rab11 antibodies. The fractions that correspond to the respective intracellular compartments are indicated above the western blots. b Bar graphs present quantitative analysis of the PS1, APP, and BACE1 levels at the plasma membrane, in total protein lysates, and the ratio of plasma membrane/total protein. Data are presented as mean ± SEM, n = 4 for PS1 in the membrane fraction, n = 8 for PS1 in total lysate, n = 5 for APP, and n = 5 for BACE1. Statistical significance was determined using the unpaired student t-test, * p < 0.05, ** p < 0.01; ***p < 0.001. PS1 presenilin 1, APP amyloid precursor protein, BACE1 β-secretase 1, Syt1 synaptotagmin 1