Fig. 7

The ability of LIX1 to regulate cell proliferation is dependent on cell density DF-1 cells infected with control RCAS(A) or RCAS(B)-LIX1 retroviruses. a Phase contrast photomicrograph at day 1. b RT-qPCR analysis of gene expression at day 1. Data were normalized to UBIQUITIN expression. Values presented are the mean ± standard deviation (SD) of n = 5 experiments. c Western blot analysis of YAP and phospho-YAP (72 kDa) protein levels. Equal loading was verified by GAPDH expression (37 kDa). Graph represents the quantification of western blot data. Values presented are the mean ± SD of n = 3 controls vs. n = 4 LIX1-expressing cell culture dishes. d Graphs represent the quantification of PH3-positive cells. A minimum of 1700 cells were analysed. Values presented are the mean ± standard error of the mean (SEM). e RT-qPCR analysis of gene expression. Values presented are the mean ± SD of six experiments. f Graphs represent the quantification of PH3-positive cells. A minimum of 1000 cells were analysed. Values presented are the mean ± SEM. g Graphs represent the quantification of cleaved CASPASE-3-positive cells. A minimum of 1000 cells were analysed. Values presented are the mean ± SEM. h Phase contrast photomicrograph of cells at day 3. i Quantification of cell growth at day 3. Values presented are the mean ± SEM of n = 10 experiments. j RT-qPCR analysis of gene expression. Values presented are the mean ± SD of n = 2 experiments. k Western blot analysis of YAP and phospho-YAP protein levels. Equal loading was verified by GAPDH expression. Graph represents the quantification of western blot data. Values presented are the mean ± SD of n = 2 experiments. Raw data for panels b, c, j and k are shown in Additional file 12. *P < 0.05; **P < 0.01; ***P < 0.001 (b, c, d, e, f and g: one-tailed Mann–Whitney test; i: two-tailed Mann–Whitney test). ns, non-significant