Fig. 4
Rescue experiments chimera. To test whether the chimeric constructs can compensate for the loss of xWnt11 and xWnt5a, 200 pg of Wnt5a/Wnt11 chimera 3.1 and Wnt11/Wnt5a 3.2 were co-injected with 2.5 pmol of an xWnt11 (Wnt11 Mo) and xWnt5a (Wnt5a Mo) morpholino (Mo) antisense oligonucleotide. a To determine the effect of the Wnt11 Mo, we counted the fraction of stage 12 embryos with open blastopore (blastopore defects). To test whether the chimeric constructs can compensate for the loss of xWnt5a we calculated (b) relative elongation and (c) relative constriction of dorsal marginal zone explants. Shown is the frequency of the indicated phenotypes. The superimposed error bars illustrate the variation between N independent experiments. N: number of biological replicates, n: number of analyzed explants, *** P < 0.001, ** P < 0.01, * P < 0.05 according to the χ2 test (a) and Fisher’s exact test (b, c)