Fig. 7
Pbk and rab11fip5 interfere with convergent extension movements. a Representative whole mount in situ hybridization for chordin of stage 12 embryos injected dorsal equatorially at the 4-cell stage with the indicated mRNAs. The dorsal overexpression of pbk and rab11fip5 results in a shorter and broader chordin expression. Bars: 500 μm. b Quantification of chordin phenotypes. *** P < 0.001 according to χ2 significance test (c). Phenotypes of dorsal marginal zone (DMZ) explants derived from pbk and rab11fip5 overexpressing embryos. Overexpression of pbk leads to broader elongated explants and overexpression of rab11fip5 results in an inhibition of elongation. Bars: 200 μm. d Quantification of elongation. Pbk does not interfere with elongation but rab11fip5 significantly inhibits elongation. e Quantification of constriction. Pbk overexpressing DMZ explants fail to constrict whereas rab11fip5 does not affect constriction. Shown is the frequency of the indicated phenotypes. In each experiment, the absolute frequency of the indicated phenotypes was normalized to the control siblings. The superimposed error bars illustrate the variation between N independent experiments. ** P < 0.01, *** P < 0.001 according to Fisher’s exact test. N: number biological replicates, n: number of analyzed embryos/explants