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Fig. 4 | BMC Biology

Fig. 4

From: Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation

Fig. 4

Abnormal alveolar epithelium and decreased type I pneumocytes in Col4a1 +/Δex41. ag co-staining of pSPC and podoplanin (PDPL). a, c, e PDPL normally lines the majority of the internal saccular surface and alveoli. b At E18.5, Col4a1 +/Δex41 PDPL lined the collapsed saccules, which are surrounded by clusters of pSPC cells. d, f At P6 and P30, the Col4a1 +/Δex41 alveolar epithelium is disorganized, as PDPL and pSPC staining shows. g Tie2-Cre; Col4a1 +/Flex41 mice pSPC cell clustering was absent. hn HOPX staining in the nucleus and cytosol of type I pneumocytes. (i, k, m) Col4a1 +/Δex41 lungs display fewer nuclear HOPX+ cells (arrows), more prominently at P6 and P30. n Tie2-Cre; Col4a1 +/Flex41 lungs show similar staining to normal lungs. Scale bars = 50 μm in ag and 200 μm in hn

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