Fig. 9

Characterization of Nasonia transcripts with unexpected responses to pRNAi. a–i Simultaneous detection of transcript of interest (red), twist (green) (to confirm the knockdown: expansion of twist in gbb1 KD and loss of twist in TollA1 KD), and DAPI (white) in blastoderm stage embryos. Changes in expression of tsg2 (a–c), Wee1 (d–f), and Nasvi2EG010608 (g–i) between wild type (a, d, f), BMP knockdown (b, e, g), and Toll knockdown (c, f, i) embryos are observed. j–o non-fluorescent detection of Nasvi2EG029763 transcripts. Dorsal (j–l) and ventral (m–o) expression is observed prior to the formation of the syncytium (j, m), through all blastoderm stages (k, n), and persists through gastrulation and germband elongation (l, o). In all frames, anterior is left and dorsal is up