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Fig. 1 | BMC Biology

Fig. 1

From: Segregation of prokaryotic magnetosomes organelles is driven by treadmilling of a dynamic actin-like MamK filament

Fig. 1

Imaging of magnetosome chain (MC) motion throughout the cell cycle. a In vivo time-lapse fluorescence microscopy of MCs by means of MamC-EGFP signal (green) in the wildtype (WT). White bars: center of EGFP signal position. Distances between bars are indicated in the first and last image. White arrowheads indicate the frame in which cytokinesis has been completed for each cell. White dotted lines: MamC-EGFP signal progression. b Kymograph displaying the MamC-EGFP signal (x-axis) over the time (y-axis) of the WT cell indicated in “A” (dashed line box). Schemes above and below depict the septum and MC position at the starting and ending point of the time-lapse. c In vivo time-lapse fluorescence microscopy of MCs in the mamK D161A strain. Star: mispositioning of the chain at cell pole. d Kymograph displaying the MamC-EGFP signal (x-axis) over the time (y-axis) of the mamK D161A cell indicated in “C” (dashed line box). Schemes above and below depict the septum and MC position at the starting and ending point of the time-lapse. Scale bars: 1 μm. Scale bars of kymographs: 500 nm. e MC cumulative displacement as a function of time in the WT (n = 24) and mamK D161A (n = 19) strains. Cumulative displacement was determined from the MamC-EGFP fluorescence signal

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