Fig. 6

Impt-1 knockdown activates SKN-1 and DAF-16 at different ages. a Average mean GFP intensity at L2 stage of the Pgst-4::GFP reporter strain treated with L4440 (control) or impt-1 RNAi since L1 (L4440, n = 92; impt-1, n = 54; *P < 0.001, unpaired Student t test). Bars are mean ± SEM. This experiment was repeated three times. b Average mean GFP intensity at different days of adulthood of the Psod-3::GFP reporter strain treated with L4440 (control) or impt-1 RNAi from L1 (L4440, n = 24–30; impt-1, n = 22–28 varying depending on the time point; *P = 0.0142 for the RNAi effect, two-way ANOVA). This is a composite of two independent experiments. c Representative images of (b) at day 3. Dashed lines delimitate the body of the worm; head and tail are indicated by arrows. d Qualitative analysis of the fraction of worms with high, medium, or low levels of nuclear DAF-16::GFP upon incubation with L4440 (control) or impt-1 RNAi from L1 (L4440, n = 25; impt-1, n = 26; χ² test). Data are presented as mean ± SEM in (a, b) or as stacked fractions in (d)