Fig. 3
From: Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis
Ablation of p53 rescues the intestinal aganglionosis of Wnt1Cre|Gem embryos. Whole-mount gut preparations of control (a), control p53 +/– (e), control p53 –/– (c), Wnt1Cre|Gem (b), Wnt1Cre|Gem p53 +/– (f–h) and Wnt1Cre|Gem p53 –/– (d) E12.5 embryos immunostained for GFP to visualise migrating ENCCs. Red arrows indicate the position of the most caudally located ENCCs in the gut preparations. Percentages in d, f, g and h represent a quantification of the analysed gut preparations that show the phenotype depicted in these panels. i, Quantification of the integrated density of GFP+ ENCCs per μm2 in the midgut of control and mutant embryos divided in four equal length bins. Two-way ANOVA followed by Fisher’s LSD multiple comparison test, *P value < 0.05, **P value < 0.01, NS: non-significant. Scale bar: (a–h) 400 μm