Fig. 5

Three-month-old iHip14 ^Δ/Δ mice have increased brain and forebrain weight, increased cortical volume, and decreased corpus callosum volume. iHip14 ^Δ/Δ mice have increased brain weight compared to control mice (a; ANOVA: p = 0.0004; N = 13–18), larger forebrain weight (b; ANOVA: p = 0.0002; N = 13–18), and unchanged cerebellum weight (c; ANOVA: p < 0.0001; N = 12–18). Brains were then sectioned and stained with NeuN to stain neurons, and striatal (d), cortical (e), and corpus callosum (f; CC) volume were determined. No change in striatal volume was observed (d; ANOVA: p = 0.43; N = 18–23) in iHip14 ^Δ/Δ mice, but there was a significant increase in cortical volume (e; ANOVA: p < 0.0001; N = 13–18) and decrease in corpus callosum volume (f; ANOVA: p < 0.0001; N = 13–18). Sections were stained with antibodies against glial fibrillary acidic protein (GFAP) or ionized calcium-binding adapter molecule 1 (IBA1) to stain astrocytes (g and i) and microglia (h and j). There was increased staining intensity of GFAP (g and i; ANOVA: p < 0.0001; N = 7–9) and IBA1 in iHip14 ^Δ/Δ cortex (h and j; ANOVA: p = 0.0001; N = 7–9)