Fig. 7

ALDH1A1 is a key oncogenic effector of AURKA. a scrambled shRNA-expressing BxPC3 and ALDH1A1 knock-down-BxPC3 cells were analyzed by FACS analysis. b ALDH1A1 promotes cell proliferation in BxPC3 cells. BxPC3, AURKA-BxPC3, ALDH1A1-BxPC3, and 3A-ALDH1A1-BxPC3 cells were plated in 96-well plates and cultured for 24, 48, and 72 h. At the end of the incubation, an MTT assay was performed. c ALDH1A1 promotes cell proliferation in Panc1 cells. d AURKA depletion decreases cell proliferation in ALDH1A1-BxPC3 cells, but not in phospho-resistant 3A-ALDH1A1-BxPC3 cells. MTT assay was performed after 48 h of transfection. The statistical significance was analyzed using two-independent-sample t test, *p < 0.05, **p < 0.01 compared to scrambled shRNA controls. e AURKA overexpression increases cell proliferation in ALDH1A1-BxPC3 cells, but not in 3A-ALDH1A1-BxPC3 cells. MTT was performed after 48 h of transfection. *p < 0.05 compared to vector-infected controls analyzed using two-independent-sample t test. f AURKA depletion decreases cell proliferation in ALDH1A1-Panc1 cells, but not in phospho-resistant 3A-ALDH1A1-Panc1 cells. g AURKA overexpression increases cell proliferation in ALDH1A1-Panc1 cells, but not in 3A-ALDH1A1-Panc1 cells. h, i ALDH1A1 promotes colony formation in a soft agar assay in (h) BxPC3 and (i) Panc1 cells. *p < 0.05 compared to vector-expressing control analyzed by two-way analysis of variance. j ALDH1A1 promotes cell motility in BxPC3 cells. **p < 0.01 compared to vector-expressing control by two-tailed Student’s t test. k AURKA depletion inhibits cell motility in ALDH1A1-BxPC3 cells, but not in phospho-resistant 3A-ALDH1A1-BxPC3 cells. **p < 0.01 compared to scrambled shRNA control by two-tailed Student’s t test. l AURKA overexpression increases cell motility in ALDH1A1-BxPC3 cells, but not in 3A-ALDH1A1-BxPC3 cells. m ALDH1A1 promotes cell motility in Panc1 cells. Chemotaxis assay was performed in ALDH1A1-Panc1 and 3A-ALDH1A1-Panc1 using Boyden chambers. These experiments were performed three independent times. Representative data are shown. Magnification, 200×. n Histogram shows mean ± SEM of three independent experiments. **p < 0.01 compared to vector-expressing control analyzed by two-way analysis of variance. o, p AURKA depletion inhibits cell motility in ALDH1A1-Panc1 cells, but not in phospho-resistant 3A-ALDH1A1-Panc1 cells. q, r AURKA overexpression increases cell motility in ALDH1A1-Panc1 cells, but not in 3A-ALDH1A1-Panc1 cells. Data used to generate the summary statistics shown in panels c, f, g, i, n, p, and r are reported in Additional file 6