Fig. 5

Expression levels of MyoC determine localisation to the inner membrane complex in the myoA knockout (KO) parasites. a Schematics of the plasmid (upper panel) and localisation of MyoC under different expression levels in both RH Δhxgprt and myoA KO (lower panel). a Constitutive overexpression (p5RT70-Ty-MyoC; left panel) and MyoC under its endogenous promoter (pMyoC-Ty-MyoC; middle panel), these constructs were randomly integrated. The endogenous MyoC locus was tagged in RH ΔKu80 via double homologous recombination (right panel). Schematic represents the final locus. Scale bar: 2 μm. b Corrected cell fluorescence intensities for different MyoC expression levels were measured using α-Ty. One-way ANOVA followed by Tukey’s post hoc test was used to compare means between groups. *** P < 0.0001, non-significance (ns) P > 0.05. c Plaque assay of myoA KO expressing the three different constructs. RH Δhxgprt was used as the control. The plaque area was measured using ImageJ with error bars representing ± standard deviation (n = 15 plaques). One-way ANOVA followed by Tukey’s post hoc test was used to compare means between groups. **** P < 0.0001, ** P < 0.001