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Fig. 2 | BMC Biology

Fig. 2

From: Rapid, precise quantification of bacterial cellular dimensions across a genomic-scale knockout library

Fig. 2

Morphometrics achieves unbiased contour extraction across a wide range of cell shapes and object types, including densely packed communities and tissues. a Contours extracted from the root-tip cells of an A. thaliana plant expressing a YFP-fusion to the membrane protein LTI6B, showing that Morphometrics is capable of segmentation of a complex tissue. Scale bar: 15 μm. b, c Contours extracted in PF mode (b) or PC mode (c) from FM4-64-labeled C. crescentus cells. d Contours extracted in PC from approximately spherical hypotonic red blood cells. e Contours extracted in PC from the budding yeast S. cerevisiae. f Contours extracted in PC from a densely packed P. aeruginosa community. g Contours extracted from transmission electron microscopy images of N. gonorrhoeae. h Contours extracted in PC from branched B. bifidum DSM20213 cells. i Contours extracted from bright-field microscopy of live zebrafish Danio rerio. j Contours extracted in IF from filamentous E. coli expressing cytoplasmic GFP

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